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Anti-ATG4C antibody [EPR15001] (ab183516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15001] to ATG4C
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ATG4C antibody [EPR15001]
    See all ATG4C primary antibodies

  • Description

    Rabbit monoclonal [EPR15001] to ATG4C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293 cells and cell lysates; Jurkat and Raji cell lysates. IHC-P: Human skeletal muscle tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ATG4C antibody [EPR15001] (ab183516)
    Western blot - Anti-ATG4C antibody [EPR15001] (ab183516)
    All lanes : Anti-ATG4C antibody [EPR15001] (ab183516) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ATG4C knockout HEK293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab183516 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab183516 Anti-ATG4C antibody [EPR15001] was shown to specifically react with ATG4C in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267324 (knockout cell lysate ab257847) was used. Wild-type and ATG4C knockout samples were subjected to SDS-PAGE. ab183516 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4C antibody [EPR15001] (ab183516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4C antibody [EPR15001] (ab183516)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ATG4C with ab183516 at 1/250 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-ATG4C antibody [EPR15001] (ab183516)
    Western blot - Anti-ATG4C antibody [EPR15001] (ab183516)
    All lanes : Anti-ATG4C antibody [EPR15001] (ab183516) at 1/20000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 52 kDa

  • Flow Cytometry - Anti-ATG4C antibody [EPR15001] (ab183516)
    Flow Cytometry - Anti-ATG4C antibody [EPR15001] (ab183516)

    Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling ATG4C with ab183516 at 1/100 dilution (green). Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control (red).

  • Anti-ATG4C antibody [EPR15001] (ab183516)
    Anti-ATG4C antibody [EPR15001] (ab183516)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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