Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13667(2)] to ATE1 - N-terminal
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ATE1 antibody [EPR13667(2)] - N-terminal
    See all ATE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13667(2)] to ATE1 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, HeLa and 293 whole cell lysate. Human fetal liver tissue lysate. Mouse brain and spleen tissue lysate. C6, Raw264.7, PC-12 and NIH/3T3 whole cell lysates. Mouse and Rat kidney tissue lysate. Rat spleen tissue lysate. IHC: Mouse liver tissue, Human hepatocellular carcinoma tissue, Rat kidney tissue. ICC/IF: HepG2 cells. Flow Cyt: HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13667(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate
    Lane 5 : Mouse kidney tissue lysate
    Lane 6 : Rat kidney tissue lysate
    Lane 7 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasmic staining on human hepatocellular carcinoma tissue is observed (Subcellular location: Nucleus and cytoplasm [UniProt]). Counter-stained with hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    Flow Cytometry - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ATE1 with ab199423 at 1/220 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/10000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate
    Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Mouse liver tissue is observed (Subcellular location: Nucleus and Cytoplasm [UniProt]) . Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa


    Exposure time: 1 minute


    Blocking and diluting buffer 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Rat kidney tissue is observed (Subcellular location: Nucleus and Cytoplasm [UniProt]) . Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/10000 dilution + Human fetal liver tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa


    Exposure time: 10 seconds


    Blocking and diluting buffer 5% NFDM/TBST

  • Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
    Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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