Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
![Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194726-anti-aspartate-beta-hydroxylase-antibody-epr10351-western-blot.jpg "Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10351] to Aspartate beta hydroxylase
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Aspartate beta hydroxylase antibody [EPR10351]
See all Aspartate beta hydroxylase primary antibodies -
Description
Rabbit monoclonal [EPR10351] to Aspartate beta hydroxylase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Aspartate beta hydroxylase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: Q12797 -
Positive control
- HeLa, A549 and HepG2 whole cell lysate (ab7900); Human colon carcinoma and pancreas tissues; HeLa cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10351 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)All lanes : Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 86 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194728-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194727-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194729-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunofluorescent analysis of HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/250 dilution (red). DAPI nuclear staining (blue).
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![Flow Cytometry - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194730-anti-aspartate-beta-hydroxylase-antibody-epr10351-flow-cytometry.jpg)
Flow Cytometry - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Flow cytometric analysis of permeabilized HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/10 dilution (red) compared to a Rabbit IgG (negative) (green).
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![Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-6-benefits-of-recombinant-antibodies.png)
Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)