Anti-Asparagine synthetase antibody (ab226413)
Key features and details
- Rabbit polyclonal to Asparagine synthetase
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Asparagine synthetase antibody
See all Asparagine synthetase primary antibodies -
Description
Rabbit polyclonal to Asparagine synthetase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human Asparagine synthetase aa 511-561. The exact sequence is proprietary.
Database link: P08243 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab226413 has not performed satisfactorily when used for WB of Asparagine Synthetase in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Asparagine Synthetase.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Asparagine synthetase antibody (ab226413)
Asparagine synthetase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226413 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226413 at 0.4 µg/ml.
Lane 1: ab226413 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
