Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ASGR2 antibody [EPR16974] (ab197032)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16974] to ASGR2
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-ASGR2 antibody [EPR16974]
See all ASGR2 primary antibodies
Description

Rabbit monoclonal [EPR16974] to ASGR2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal liver lysate, HepG2 whole cell lysate, Human liver tissue.
General notes
Previously labelled as Asialoglycoprotein Receptor 2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16974
Isotype

IgG
Research areas

Western blot - Anti-ASGR2 antibody [EPR16974] (ab197032)

All lanes : Anti-ASGR2 antibody [EPR16974] (ab197032) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 5 : HeLa (Human epithetial cells from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 35 kDa
Observed band size: 46 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and diluting buffer was 5% NFDM/TBST.
The observed MW is consistent with that described in the literature (PMID:3040719).
Western blot - Anti-ASGR2 antibody [EPR16974] (ab197032)

Anti-ASGR2 antibody [EPR16974] (ab197032) at 1/2000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 35 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and diluting buffer was 5% NFDM/TBST.
The observed MW is consistent with that described in the literature (PMID:3040719).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16974] (ab197032)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ASGR2 with ab197032 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cell membrane staining on Human liver tissue is observed; Uniprot suggests Cell membrane as subcellular location. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16974] (ab197032)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue (negative control tissue) using ab197032 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. No staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-ASGR2 antibody [EPR16974] (ab197032)

ASGR2 was immunoprecipitated from Human fetal liver with ab197032 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab197032 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human fetal liver. Lane 2: Rabbit monoclonal IgG (ab172730) instead of ab197032 in Human fetal liver.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-ASGR2 antibody [EPR16974] (ab197032)

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