Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)
![Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)](https://www.abcam.com/ps/products/232/ab232664/Images/ab232664-3-ab197032237651ab197032IP.jpg "Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16974] to ASGR2 - BSA and Azide free
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-ASGR2 antibody [EPR16974] - BSA and Azide free
See all ASGR2 primary antibodies -
Description
Rabbit monoclonal [EPR16974] to ASGR2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human liver tissue.
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General notes
ab232664 is the carrier-free version of ab197032 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab232664 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Previously labelled as Asialoglycoprotein Receptor 2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16974 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)
ASGR2 was immunoprecipitated from Human fetal liver with ab197032 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab197032 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human fetal liver. Lane 2: Rabbit monoclonal IgG (ab172730) instead of ab197032 in Human fetal liver.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197032).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)](https://www.abcam.com/ps/products/232/ab232664/Images/ab232664-2-ab197032237647ab197032IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue (negative control tissue) using ab197032 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. No staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197032).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)](https://www.abcam.com/ps/products/232/ab232664/Images/ab232664-309010-118.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ASGR2 with ab197032 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cell membrane staining on human liver tissue is observed; Uniprot suggests Cell membrane as subcellular location. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197032).
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![Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)](https://www.abcam.com/ps/products/232/ab232664/Images/ab232664-4-benefits-of-recombinant-antibodies.png)
Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)
