Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16974] to ASGR2 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ASGR2 antibody [EPR16974] - BSA and Azide free
    See all ASGR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16974] to ASGR2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver tissue.

  • General notes

    ab232664 is the carrier-free version of ab197032 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232664 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Asialoglycoprotein Receptor 2.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16974

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)
    Immunoprecipitation - Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)

    ASGR2 was immunoprecipitated from Human fetal liver with ab197032 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab197032 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human fetal liver. Lane 2: Rabbit monoclonal IgG (ab172730) instead of ab197032 in Human fetal liver.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue (negative control tissue) using  ab197032 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. No staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ASGR2 with ab197032 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cell membrane staining on human liver tissue is observed; Uniprot suggests Cell membrane as subcellular location. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197032).

     

  • Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)
    Anti-ASGR2 antibody [EPR16974] - BSA and Azide free (ab232664)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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