Anti-ARL 1 antibody [EPR10595] (ab155982)
![Anti-ARL 1 antibody [EPR10595] (ab155982)](https://www.abcam.com/ps/products/155/ab155982/Images/ARL-1-Primary-antibodies-ab155982-2.jpg "Anti-ARL 1 antibody [EPR10595] (ab155982)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10595] to ARL 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-ARL 1 antibody [EPR10595]
See all ARL 1 primary antibodies -
Description
Rabbit monoclonal [EPR10595] to ARL 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide, corresponding to residues in Human ARL 1 (UniProt: P40616).
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Positive control
- HepG2, MCF7, HeLa and K562 cell lysate; Human liver and pancreas tissues. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCo2
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10595 -
Isotype
IgG
Images
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Western blot - Anti-ARL 1 antibody [EPR10595] (ab155982)All lanes : Anti-ARL 1 antibody [EPR10595] (ab155982) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 20 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-ARL 1 antibody [EPR10595] (ab155982)](https://www.abcam.com/ps/products/155/ab155982/Images/ab155982-210252-ab155982caco2p10ugmledit.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ARL 1 antibody [EPR10595] (ab155982)ICC/IF image of ab155982 stained CaCo2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab155982 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)](https://www.abcam.com/ps/products/155/ab155982/Images/ab155982-8-YCAR10595-ARL1-prostate.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)ab155982 showing +ve staining in Human normal prostate.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)](https://www.abcam.com/ps/products/155/ab155982/Images/ab155982-7-YCAR10595-ARL1-gastric-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)ab155982 showing +ve staining in Human gastric adenocarcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)](https://www.abcam.com/ps/products/155/ab155982/Images/ab155982-6-YCAR10595-ARL1-endometrial-adenocarcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)ab155982 showing +ve staining in Human endometrial carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)](https://www.abcam.com/ps/products/155/ab155982/Images/ab155982-5-YCAR10595-ARL1-brain.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)ab155982 showing +ve staining in Human normal brain.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)](https://www.abcam.com/ps/products/155/ab155982/Images/ab155982-4-YCAR10595-ARL1-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)ab155982 showing +ve staining in Human normal liver.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)](https://www.abcam.com/ps/products/155/ab155982/Images/ARL-1-Primary-antibodies-ab155982-7.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ARL 1 with ab155982 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)](https://www.abcam.com/ps/products/155/ab155982/Images/ARL-1-Primary-antibodies-ab155982-8.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (ab155982)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling ARL 1 with ab155982 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-ARL 1 antibody [EPR10595] (ab155982)](https://www.abcam.com/ps/products/155/ab155982/Images/ab155982-10-benefits-of-recombinant-antibodies.png)
Anti-ARL 1 antibody [EPR10595] (ab155982)