Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARF4 antibody [EPR12133(B)] (ab171746)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12133(B)] to ARF4
  • Suitable for: WB, ICC/IF, IHC-P, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-ARF4 antibody [EPR12133(B)]
    See all ARF4 primary antibodies

  • Description

    Rabbit monoclonal [EPR12133(B)] to ARF4

  • Host species

    Rabbit

  • Specificity

    May cross react with family members, immunogen has 86% homology with ARF5

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ARF4 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P18085

  • Positive control

    • HT-29, HepG2, K562 and HeLa whole cell lysate (ab150035); Human kidney and testis tissues; HepG2 cells; permeabilized K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12133(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ARF4 antibody [EPR12133(B)] (ab171746)
    Western blot - Anti-ARF4 antibody [EPR12133(B)] (ab171746)
    All lanes : Anti-ARF4 antibody [EPR12133(B)] (ab171746) at 1/10000 dilution

    Lane 1 : HT-29 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 21 kDa

  • Immunoprecipitation - Anti-ARF4 antibody [EPR12133(B)] (ab171746)
    Immunoprecipitation - Anti-ARF4 antibody [EPR12133(B)] (ab171746)

    Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cell lysate or (Lane 2) 1X PBS (negative control) using ab171746 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF4 antibody [EPR12133(B)] (ab171746)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF4 antibody [EPR12133(B)] (ab171746)

    Immunohistochemistry of paraffin-embedded Human testis tissue labeling ARF4 using ab171746 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF4 antibody [EPR12133(B)] (ab171746)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF4 antibody [EPR12133(B)] (ab171746)

    Immunohistochemistry of paraffin-embedded Human kidney tissue labeling ARF4 using ab171746 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ARF4 antibody [EPR12133(B)] (ab171746)
    Immunocytochemistry/ Immunofluorescence - Anti-ARF4 antibody [EPR12133(B)] (ab171746)

    Immunofluorescence analysis of HepG2 cells labeling ARF4 using ab171746 at 1/100 dilution (green). DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-ARF4 antibody [EPR12133(B)] (ab171746)
    Flow Cytometry - Anti-ARF4 antibody [EPR12133(B)] (ab171746)

    Flow cytometric analysis of permeabilized K562 cells labeling ARF4  using ab171746 (red) at 1/10 dilution, or a rabbit IgG (negative) (green).

  • Anti-ARF4 antibody [EPR12133(B)] (ab171746)
    Anti-ARF4 antibody [EPR12133(B)] (ab171746)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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