Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ARF4 antibody [EPR12133(B)] (ab171746)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12133(B)] to ARF4
Suitable for: WB, ICC/IF, IHC-P, IP, Flow Cyt
Reacts with: Human

Product name

Anti-ARF4 antibody [EPR12133(B)]
See all ARF4 primary antibodies
Description

Rabbit monoclonal [EPR12133(B)] to ARF4
Host species

Rabbit
Specificity

May cross react with family members, immunogen has 86% homology with ARF5

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human ARF4 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P18085
Positive control
- HT-29, HepG2, K562 and HeLa whole cell lysate (ab150035); Human kidney and testis tissues; HepG2 cells; permeabilized K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12133(B)
Isotype

IgG
Research areas

Western blot - Anti-ARF4 antibody [EPR12133(B)] (ab171746)

All lanes : Anti-ARF4 antibody [EPR12133(B)] (ab171746) at 1/10000 dilution

Lane 1 : HT-29 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 21 kDa
Immunoprecipitation - Anti-ARF4 antibody [EPR12133(B)] (ab171746)

Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cell lysate or (Lane 2) 1X PBS (negative control) using ab171746 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF4 antibody [EPR12133(B)] (ab171746)

Immunohistochemistry of paraffin-embedded Human testis tissue labeling ARF4 using ab171746 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF4 antibody [EPR12133(B)] (ab171746)

Immunohistochemistry of paraffin-embedded Human kidney tissue labeling ARF4 using ab171746 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-ARF4 antibody [EPR12133(B)] (ab171746)

Immunofluorescence analysis of HepG2 cells labeling ARF4 using ab171746 at 1/100 dilution (green). DAPI nuclear staining (blue).
Flow Cytometry - Anti-ARF4 antibody [EPR12133(B)] (ab171746)

Flow cytometric analysis of permeabilized K562 cells labeling ARF4 using ab171746 (red) at 1/10 dilution, or a rabbit IgG (negative) (green).
Anti-ARF4 antibody [EPR12133(B)] (ab171746)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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