Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3747] to Aquaporin 5
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Aquaporin 5 antibody [EPR3747]
    See all Aquaporin 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR3747] to Aquaporin 5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Aquaporin 5 aa 200 to the C-terminus. The exact sequence is proprietary.
    (Peptide available as ab214008)

  • Positive control

    • IHC-P: Human colon, lung and stomach tissue. WB: Fetal lung lysate. SW480 cell lysate. Flow Cyt: SW480 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3747

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320) Yin et al PLoS Genet. 2015 May 15;11(5):e1005242. doi: 10.1371/journal.pgen.1005242. eCollection 2015 May. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Comparison of non-diseased human lung (left panel) and Type I PPB (right panel).

    Immunostaining for the distal lung Type I pneumocyte marker Aquaporin 5 using ab92320. Aquaorin 5 was expressed in distal alveoli of non-diseased lung tissue and in Type I PPB associated epithelium.

    The human Type I Pleuropulmonary Blastoma tissue samples, formalin fixed and paraffin embedded, were obtained through the genetic studies tissue bank of the International PPB registry.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

    ab92320 showing positive staining in human colon tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

    ab92320 showing positive staining in human stomach tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

    ab92320 at 1/100 dilution, staining Aquaporin 5 in paraffin embedded human lung tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

    ab92320 showing negative staining in human brain tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)
    Western blot - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)
    All lanes : Anti-Aquaporin 5 antibody [EPR3747] (ab92320) at 1/1000 dilution

    Lane 1 : Fetal lung lysate
    Lane 2 : SW480 (Human colorectal adenocarcinoma cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 28 kDa

  • Flow Cytometry - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)
    Flow Cytometry - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

    Flow cytometry analysis of SW480 (Human colorectal adenocarcinoma cell line) cells labeling with unpurified ab92320 at 1/200 dilution (1 µg/ml) (Red).

    Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Goat anti rabbit IgG Alexa Fluor® 488(ab150077) at a 1/2000 dilution, was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as a isotype control. Cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Anti-Aquaporin 5 antibody [EPR3747] (ab92320)
    Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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