Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸

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Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3747] to Aquaporin 5
Suitable for: Flow Cyt, WB, IHC-P
Reacts with: Human

Product name

Anti-Aquaporin 5 antibody [EPR3747]
See all Aquaporin 5 primary antibodies
Description

Rabbit monoclonal [EPR3747] to Aquaporin 5
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Aquaporin 5 aa 200 to the C-terminus. The exact sequence is proprietary.
(Peptide available as ab214008)
Positive control
- IHC-P: Human colon, lung and stomach tissue. WB: Fetal lung lysate. SW480 cell lysate. Flow Cyt: SW480 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3747
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320) Yin et al PLoS Genet. 2015 May 15;11(5):e1005242. doi: 10.1371/journal.pgen.1005242. eCollection 2015 May. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Comparison of non-diseased human lung (left panel) and Type I PPB (right panel).

Immunostaining for the distal lung Type I pneumocyte marker Aquaporin 5 using ab92320. Aquaorin 5 was expressed in distal alveoli of non-diseased lung tissue and in Type I PPB associated epithelium.

The human Type I Pleuropulmonary Blastoma tissue samples, formalin fixed and paraffin embedded, were obtained through the genetic studies tissue bank of the International PPB registry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

ab92320 showing positive staining in human colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

ab92320 showing positive staining in human stomach tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

ab92320 at 1/100 dilution, staining Aquaporin 5 in paraffin embedded human lung tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

ab92320 showing negative staining in human brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

All lanes : Anti-Aquaporin 5 antibody [EPR3747] (ab92320) at 1/1000 dilution

Lane 1 : Fetal lung lysate
Lane 2 : SW480 (Human colorectal adenocarcinoma cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 28 kDa
Flow Cytometry - Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

Flow cytometry analysis of SW480 (Human colorectal adenocarcinoma cell line) cells labeling with unpurified ab92320 at 1/200 dilution (1 µg/ml) (Red).

Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Goat anti rabbit IgG Alexa Fluor^® 488(ab150077) at a 1/2000 dilution, was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as a isotype control. Cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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