Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ENT1 antibody [EPR20556] (ab223851)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20556] to ENT1
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ENT1 antibody [EPR20556]
    See all ENT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20556] to ENT1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MDA-MB-435S, U937 and HeLa whole cell lysates; Human fetal liver and fetal spleen lysates. IHC-P: Human gallbladder adenocarcinoma and pancreatic carcinoma tissues; Mouse liver tissue; Rat colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20556

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ENT1 antibody [EPR20556] (ab223851)
    Western blot - Anti-ENT1 antibody [EPR20556] (ab223851)
    Lanes 1-3 : Anti-ENT1 antibody [EPR20556] (ab223851) at 1/5000 dilution
    Lanes 4-5 : Anti-ENT1 antibody [EPR20556] (ab223851) at 1/1000 dilution

    Lane 1 : MDA-MB-435S (human ductal carcinoma cell line) whole cell lysate at 10 µg
    Lane 2 : U937 (human histiocytic lymphoma cell line) whole cell lysate at 10 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
    Lane 4 : Human fetal liver lysate at 20 µg
    Lane 5 : Human fetal spleen lysate at 20 µg

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 4-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 50 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



     

    Exposure time : Lanes 1,4: 3 minutes;  Lane 2: 1 minute; Lanes 3,5: 15 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 12611914; PMID:10085223).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [EPR20556] (ab223851)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [EPR20556] (ab223851)

    Immunohistochemical analysis of paraffin-embedded human gallbladder adenocarcinoma tissue labeling ENT1 with ab223851 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in human gallbladder adenocarcinoma (PMID:26266900) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [EPR20556] (ab223851)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [EPR20556] (ab223851)

    Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labeling ENT1 with ab223851 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in pancreatic cancer (PMID:21913012) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [EPR20556] (ab223851)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [EPR20556] (ab223851)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ENT1 with ab223851 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in mouse liver (PMID: 17761511) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [EPR20556] (ab223851)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [EPR20556] (ab223851)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling ENT1 with ab223851 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in rat colon epithelium and endothelium (PMID:17761511) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ENT1 antibody [EPR20556] (ab223851)
    Anti-ENT1 antibody [EPR20556] (ab223851)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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