Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ENT1 antibody [SP120] - BSA and Azide free (ab240258)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP120] to ENT1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ENT1 antibody [SP120] - BSA and Azide free
    See all ENT1 primary antibodies

  • Description

    Rabbit monoclonal [SP120] to ENT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human Pancreas, Human tonsil, Human pancreas, and Human colon carcinoma tissue; Flow Cyt (intra): HepG2 cells; ICC: HAP1 cells. WB: HAP1 cell lysate.

  • General notes

    ab240258 is the carrier-free version of ab182023.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP120

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [SP120] - BSA and Azide free (ab240258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [SP120] - BSA and Azide free (ab240258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling ENT1 with Purified ab182023 at 1/100 dilution (0.77 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182023)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [SP120] - BSA and Azide free (ab240258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [SP120] - BSA and Azide free (ab240258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling ENT1 with Purified ab182023 at 1/100 dilution (0.77 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182023)
  • Flow Cytometry - Anti-ENT1 antibody [SP120] - BSA and Azide free (ab240258)
    Flow Cytometry - Anti-ENT1 antibody [SP120] - BSA and Azide free (ab240258)

    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ENT1 with purified ab182023 at 1/50 dilution (10.42 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182023).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [SP120] - BSA and Azide free (ab240258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENT1 antibody [SP120] - BSA and Azide free (ab240258)

    Immunohistochemical analysis of formalin-fixed paraffin-embedded Human pancreas tissue labeling ENT1 with ab182023.

    his data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab182023).

  • Anti-ENT1 antibody [SP120] - BSA and Azide free (ab240258)
    Anti-ENT1 antibody [SP120] - BSA and Azide free (ab240258)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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