Anti-APPBP1 antibody [EPR15732] (ab187142)
![Anti-APPBP1 antibody [EPR15732] (ab187142)](https://www.abcam.com/ps/products/187/ab187142/Images/ab187142-220364-anti-appbp1-antibody-epr15732-western-blot.jpg "Anti-APPBP1 antibody [EPR15732] (ab187142)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15732] to APPBP1
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Rat, Human
Overview
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Product name
Anti-APPBP1 antibody [EPR15732]
See all APPBP1 primary antibodies -
Description
Rabbit monoclonal [EPR15732] to APPBP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP HumanWB RatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, A431, Human fetal muscle, Rat brain, Rat heart, Rat spleen and Human fetal brain lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15732 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)All lanes : Anti-APPBP1 antibody [EPR15732] (ab187142) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Human fetal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
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![Flow Cytometry - Anti-APPBP1 antibody [EPR15732] (ab187142)](https://www.abcam.com/ps/products/187/ab187142/Images/ab187142-220366-anti-appbp1-antibody-epr15732-flow-cytometry.jpg)
Flow Cytometry - Anti-APPBP1 antibody [EPR15732] (ab187142)Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling APPBP1 with ab187142 at 1/120 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (ab187142)](https://www.abcam.com/ps/products/187/ab187142/Images/ab187142-220367-anti-appbp1-antibody-epr15732-immunoprecipitation.jpg)
Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (ab187142)Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab187142 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)](https://www.abcam.com/ps/products/187/ab187142/Images/ab187142-220365-anti-appbp1-antibody-epr15732-western-blot.jpg)
Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)All lanes : Anti-APPBP1 antibody [EPR15732] (ab187142) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
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![Anti-APPBP1 antibody [EPR15732] (ab187142)](https://www.abcam.com/ps/products/187/ab187142/Images/ab187142-5-benefits-of-recombinant-antibodies.png)
Anti-APPBP1 antibody [EPR15732] (ab187142)