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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ub-like Proteins

Anti-KPC2 antibody (ab61596)

Price and availability

264 681 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-KPC2 antibody (ab61596)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to KPC2
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-KPC2 antibody
    See all KPC2 primary antibodies
  • Description

    Rabbit polyclonal to KPC2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human KPC2 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101415, ab75268)

  • Positive control

    • This antibody gave a positive signal in Human Liver Tissue Lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ub-like Proteins
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab61596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Chicken
Zebrafish
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).

Target

  • Relevance

    KPC2 (Kip1 ubiquitylation-promoting complex 2) together with KPC1 forms the ubiquitin ligase KPC, which regulates degradation of the cyclin-dependent kinase inhibitor p27 at the G1 phase of the cell cycle. KPC2 contains a ubiquitin-like (UBL) domain, two ubiquitin-associated (UBA) domains, and a heat shock chaperonin-binding (STI1) domain.
  • Cellular localization

    Cytoplasmic
  • Database links

    • Entrez Gene: 10422 Human
    • Entrez Gene: 98766 Mouse
    • Entrez Gene: 362087 Rat
    • Omim: 608129 Human
    • SwissProt: Q9BSL1 Human
    • SwissProt: Q8VDI7 Mouse
    • SwissProt: Q5XIR9 Rat
    • Unigene: 9194 Human
    • Unigene: 247824 Mouse
    • Unigene: 6512 Rat
    see all
  • Alternative names

    • 1110033G07Rik antibody
    • AA407978 antibody
    • E3 ubiquitin-protein ligase subunit KPC2 antibody
    • GBDR1 antibody
    • Glialblastoma cell differentiation-related protein 1 antibody
    • Glialblastoma differentiation-related protein 1 antibody
    • Kip1 ubiquitination-promoting complex protein 2 antibody
    • kpc 2 antibody
    • Kpc2 antibody
    • OTTHUMP00000022563 antibody
    • Putative glialblastoma cell differentiation-related protein antibody
    • RP11-432J22.3 antibody
    • UBA domain containing 1 antibody
    • UBA domain-containing protein 1 antibody
    • UBAC 1 antibody
    • UBAC1 antibody
    • Ubadc1 antibody
    • Ubiquitin associated domain containing 1 antibody
    • Ubiquitin-associated domain-containing protein 1 antibody
    see all

Images

  • Western blot - Anti-KPC2 antibody (ab61596)
    Western blot - Anti-KPC2 antibody (ab61596)
    Anti-KPC2 antibody (ab61596) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Predicted band size: 45 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab61596? Please let us know so that we can cite the reference in this datasheet.

    ab61596 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-KPC2 antibody (ab61596)
      Western blot - Anti-KPC2 antibody (ab61596)
      Anti-KPC2 antibody (ab61596) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

      Predicted band size: 45 kDa
      Observed band size: 40 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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