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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ub-like Proteins

Anti-KPC2 antibody - C-terminal (ab177519)

Anti-KPC2 antibody - C-terminal (ab177519)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to KPC2 - C-terminal
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-KPC2 antibody - C-terminal
    See all KPC2 primary antibodies
  • Description

    Rabbit polyclonal to KPC2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human KPC2 aa 325-375 (C terminal). The exact sequence is proprietary. NP_057256.2
    Sequence:

    LLGDRK PSPEELDKGI DPDSPLFQAI LDNPVVQLGL TNPKTLLAFE DMLEN


    Database link: Q9BSL1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, HeLa, Jurkat and TCMK1 whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ub-like Proteins
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin

Images

  • Western blot - Anti-KPC2 antibody - C-terminal (ab177519)
    Western blot - Anti-KPC2 antibody - C-terminal (ab177519)
    All lanes : Anti-KPC2 antibody - C-terminal (ab177519) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Anti-rabbit HRP conjugated antibody

    Developed using the ECL technique.

    Predicted band size: 45 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-KPC2 antibody - C-terminal (ab177519)
    Immunoprecipitation - Anti-KPC2 antibody - C-terminal (ab177519)
    All lanes : Anti-KPC2 antibody - C-terminal (ab177519) at 1 µg/ml

    Lane 1 : 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated using ab177519 at 6 µg/mg lysate.
    Lane 2 : 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated using Control IgG at 6 µg/mg lysate.

    Secondary
    All lanes : Anti-rabbit HRP conjugated antibody

    Developed using the ECL technique.

    Exposure time: 30 seconds

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: ICC/IF, IHC-P, WB

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    Applications: WB

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