Anti-Apolipoprotein E antibody [D6E10] (ab1906)
Key features and details
- Mouse monoclonal [D6E10] to Apolipoprotein E
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Apolipoprotein E antibody [D6E10]
See all Apolipoprotein E primary antibodies -
Description
Mouse monoclonal [D6E10] to Apolipoprotein E -
Host species
Mouse -
Specificity
Mouse reactivity: Please be aware that we have received positive as well as negative feedback for reactivity of this antibody with mouse samples. The antibody is not being batch-tested in the mouse samples. Anti-Apolipoprotein E antibody [D6E10] recognizes the E2, E3 and E4 isoforms of apolipoprotein E. It was raised against a peptide sequence corresponding to aa 141-160 of human Apo-E. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human -
Immunogen
Synthetic peptide corresponding to Apolipoprotein E aa 100-200.
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General notes
This product was changed from ascites to tissue culture supernatant on 2nd February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
D6E10 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Alzheimer’s disease brain tissue labelling Apolipoprotein E with ab1906. The tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Antigen retrieval was performed using Sodium Citrate H.I.E.R. Counterstained with hematoxylin. The image was captured with a 40X objective. Scale bar: 50 µm.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling Apolipoprotein E with ab1906. The tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. Antigen retrieval was performed using Sodium Citrate H.I.E.R. Counterstained with hematoxylin. The image was captured with a 40X objective. Scale bar: 50 µm.
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ab1906 staining Apolipoprotein E in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/200 in TBS/BSA/azide) for 2 hours at 21°C. An undiluted biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.