Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19507] to Annexin A10/ANXA10
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Annexin A10/ANXA10 antibody [EPR19507]
    See all Annexin A10/ANXA10 primary antibodies

  • Description

    Rabbit monoclonal [EPR19507] to Annexin A10/ANXA10

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat stomach lysates; BxPC-3 whole cell lysate. IHC-P: Human stomach, gastric cancer and bladder cancer tissues; mouse and rat stomach tissues.

  • General notes

     This product was previously labelled as Annexin A10

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)
    Western blot - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)
    All lanes : Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656) at 1/1000 dilution

    Lane 1 : Human stomach lysate
    Lane 2 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 37 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 5 seconds; Lane 2: 3 minutes.

    The expression profile observed is consistent with what has been described in the literature (PMID: 24024557).

  • Western blot - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)
    Western blot - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)
    All lanes : Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656) at 1/1000 dilution

    Lane 1 : Mouse stomach lysate
    Lane 2 : Rat stomach lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 37 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 seconds; Lane 2: 10 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Annexin A10/ANXA10 with ab213656 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on human stomach is observed [PMID:24024557]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Annexin A10/ANXA10 with ab213656 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on human gastric cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Annexin A10/ANXA10 with ab213656 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on human bladder cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Annexin A10/ANXA10 with ab213656 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on mouse stomach is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Annexin A10/ANXA10 with ab213656 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on rat stomach is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)
    Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

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