Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19507] to Annexin A10/ANXA10
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Annexin A10/ANXA10 antibody [EPR19507]
See all Annexin A10/ANXA10 primary antibodies
Description

Rabbit monoclonal [EPR19507] to Annexin A10/ANXA10
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, mouse and rat stomach lysates; BxPC-3 whole cell lysate. IHC-P: Human stomach, gastric cancer and bladder cancer tissues; mouse and rat stomach tissues.
General notes
This product was previously labelled as Annexin A10

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19507
Isotype

IgG
Research areas

Western blot - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

All lanes : Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656) at 1/1000 dilution

Lane 1 : Human stomach lysate
Lane 2 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 37 kDa
Observed band size: 35 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 5 seconds; Lane 2: 3 minutes.

The expression profile observed is consistent with what has been described in the literature (PMID: 24024557).
Western blot - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

All lanes : Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656) at 1/1000 dilution

Lane 1 : Mouse stomach lysate
Lane 2 : Rat stomach lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 3 seconds; Lane 2: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Annexin A10/ANXA10 with ab213656 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on human stomach is observed [PMID:24024557]. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Annexin A10/ANXA10 with ab213656 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on human gastric cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Annexin A10/ANXA10 with ab213656 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on human bladder cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Annexin A10/ANXA10 with ab213656 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on mouse stomach is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Annexin A10/ANXA10 with ab213656 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on rat stomach is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Annexin A10/ANXA10 antibody [EPR19507] (ab213656)

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