Anti-ANKRD57 antibody (ab99380)
Key features and details
- Rabbit polyclonal to ANKRD57
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ANKRD57 antibody
See all ANKRD57 primary antibodies -
Description
Rabbit polyclonal to ANKRD57 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human ANKRD57 aa 75-125. The exact sequence is proprietary. (NP_075392.2).
Database link: Q53LP3 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-ANKRD57 antibody (ab99380)All lanes : Anti-ANKRD57 antibody (ab99380) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-ANKRD57 antibody (ab99380)ANKRD57 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab99380 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab99380 at 0.1 µg/ml.
Lane 1: ab99380 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
