Anti-Angiopoietin 1 antibody (ab95230)
Key features and details
- Rabbit polyclonal to Angiopoietin 1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Angiopoietin 1 antibody
See all Angiopoietin 1 primary antibodies -
Description
Rabbit polyclonal to Angiopoietin 1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Sheep, Cow, Dog, Human, Pig
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab109637) -
Positive control
- This antibody gave a positive signal in both E10 and E11 Mouse embryonic brain tissue lysates and MEF1 cell line.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 57 kDa). Target
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Function
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 282140 Cow
- Entrez Gene: 403656 Dog
- Entrez Gene: 284 Human
- Entrez Gene: 11600 Mouse
- Entrez Gene: 397009 Pig
- Entrez Gene: 89807 Rat
- Omim: 601667 Human
- SwissProt: O18920 Cow
see all -
Alternative names
- AGP 1 antibody
- AGP1 antibody
- AGPT antibody
see all
Images
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Western blot - Anti-Angiopoietin 1 antibody (ab95230)Anti-Angiopoietin 1 antibody (ab95230) at 1 µg/ml + E10 Mouse Embryo Brain Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 53 kDa could potentially be a cleaved form of Angiopoietin 1 due to the presence of a 19 amino acid signal peptide. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin 1 antibody (ab95230)ICC/IF image of ab95230 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95230, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-Angiopoietin 1 antibody (ab95230)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Angiopoietin 1 antibody (ab95230) at 1/500 dilution
Lane 1 : Sheep fetal extra-embryonic membrane purified protein at 50 µg
Lane 2 : Sheep fetal extra-embryonic membrane purified protein at 30 µg
Lane 3 : Sheep fetal extra-embryonic membrane purified protein at 20 µg
Lane 4 : Sheep fetal extra-embryonic membrane purified protein at 10 µg
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 90 seconds
Protocols
Datasheets and documents
References (10)
ab95230 has been referenced in 10 publications.
- Severe N et al. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping. Cell Stem Cell 25:570-583.e7 (2019). PubMed: 31279774
- Zhang X et al. Matrine inhibits the development and progression of ovarian cancer by repressing cancer associated phosphorylation signaling pathways. Cell Death Dis 10:770 (2019). PubMed: 31601793
- Lu Y et al. Transcriptome Profiling of Neovascularized Corneas Reveals miR-204 as a Multi-target Biotherapy Deliverable by rAAVs. Mol Ther Nucleic Acids 10:349-360 (2018). WB . PubMed: 29499946
- Gram A et al. Angiopoietin expression in ovine corpora lutea during the luteal phase: Effects of nutrition, arginine and follicle stimulating hormone. Gen Comp Endocrinol 269:131-140 (2018). PubMed: 30195024
- Lee YJ et al. Hyperhomocysteinemia Causes Chorioretinal Angiogenesis with Placental Growth Factor Upregulation. Sci Rep 8:15755 (2018). PubMed: 30361676
- Lee YJ et al. Homocysteine Facilitates Prominent Polygonal Angiogenetic Networks of a Choroidal Capillary Sprouting Model. Invest Ophthalmol Vis Sci 58:4332–4343 (2017). PubMed: 28806447
- Mohammadi E et al. Endothelial juxtaposition of distinct adult stem cells activates angiogenesis signaling molecules in endothelial cells. Cell Tissue Res 362:597-609 (2015). PubMed: 26068799
- Coleson MP et al. Human chorionic gonadotropin increases serum progesterone, number of corpora lutea and angiogenic factors in pregnant sheep. Reproduction 150:43-52 (2015). PubMed: 25861798
- Duran JM et al. Bone-derived stem cells repair the heart after myocardial infarction through transdifferentiation and paracrine signaling mechanisms. Circ Res 113:539-52 (2013). ICC/IF, WB ; Mouse . PubMed: 23801066
- Lv J et al. The chinese herbal decoction danggui buxue tang inhibits angiogenesis in a rat model of liver fibrosis. Evid Based Complement Alternat Med 2012:284963 (2012). Rat . PubMed: 22927877
Images
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Western blot - Anti-Angiopoietin 1 antibody (ab95230)Anti-Angiopoietin 1 antibody (ab95230) at 1 µg/ml + E10 Mouse Embryo Brain Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 53 kDa could potentially be a cleaved form of Angiopoietin 1 due to the presence of a 19 amino acid signal peptide. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin 1 antibody (ab95230)ICC/IF image of ab95230 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95230, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Western blot - Anti-Angiopoietin 1 antibody (ab95230) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Angiopoietin 1 antibody (ab95230) at 1/500 dilution
Lane 1 : Sheep fetal extra-embryonic membrane purified protein at 50 µg
Lane 2 : Sheep fetal extra-embryonic membrane purified protein at 30 µg
Lane 3 : Sheep fetal extra-embryonic membrane purified protein at 20 µg
Lane 4 : Sheep fetal extra-embryonic membrane purified protein at 10 µg
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 90 seconds
