Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Androgen Receptor antibody [EP670Y] (ab52615)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP670Y] to Androgen Receptor
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Androgen Receptor antibody [EP670Y]
See all Androgen Receptor primary antibodies
Description

Rabbit monoclonal [EP670Y] to Androgen Receptor
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide within Human Androgen Receptor aa 900 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P10275
(Peptide available as ab174742)
Positive control
- WB: PC3 whole cell lysate (ab3954), LnCaP and 22Rv1 cell lysate and mouse and rat testis tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP670Y
Isotype

IgG
Research areas

Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)

All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/1000 dilution

Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 99 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Diluting buffer: 5% NFDM/TBST

Loading control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)
Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)

All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/1000 dilution

Lane 1 : LnCAP whole cell lysate (positive control)
Lane 2 : PC3 whole cell lysate (negative control)

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size: 99 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.

Lanes 1 - 2: Merged signal (red and green). Green - ab52615 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52615 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.
Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)

All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/5000 dilution (Purified)

Lane 1 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
Lane 2 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol here (downloadable copy).
Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)

Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/10000 dilution (unpurified) + LnCaP cell lysate at 10 µg

Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 99 kDa
Observed band size: 99 kDa
Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)

All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/2000 dilution (purified)

Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Anti-Androgen Receptor antibody [EP670Y] (ab52615)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com