All lanes : Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225) at 1/1000 dilution

Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lane 3 : Mouse testis lysate
Lane 4 : Rat testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?


Exposure time: 23 seconds

Blocking/Diluting buffer: 5% NFDM/TBST

Loading Control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)

All lanes : Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225) at 1.55 µg

Lane 1 : LnCAP (Human prostate carcinoma epithelial cell) cell lysate
Lane 2 : DU 145 (Human prostate carcinoma epithelial cell) cell lysate
Lane 3 : Mouse testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

Predicted band size: 99 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?



Blocking/Diluting buffer and concentration: 5% NFDM /TBST

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat testis tissue sections labeling Androgen Receptor with Purified ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Androgen Receptor with Purified ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate cancer tissue sections labeling Androgen Receptor with Purified ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human uterus tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human breast tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human cervix tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human placenta tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human prostate tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human testis tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.