Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Amino-terminal enhancer of split/AES antibody [EPR8385]
    See all Amino-terminal enhancer of split/AES primary antibodies

  • Description

    Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human Amino-terminal enhancer of split/AES aa 150-250 (C terminal).
    Database link: Q08117

  • Positive control

    • WB: HCT116, 293T, HeLa, PC3 and Jurkat cell lysates. ICC: HeLa cells.

  • General notes

     

     

     This product was previously labelled as Amino-terminal enhancer of split

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
    Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
    All lanes : Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060) at 1/1000 dilution

    Lane 1 : Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
    Lane 2 : AES knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : PC3 (Human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab137060 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab137060 Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] was shown to specifically react with Amino-terminal enhancer of split/AES in wild-type HCT cells. Loss of signal was observed when knockout cell line ab266886 (knockout cell lysate ab257818) was used. Wild-type and Amino-terminal enhancer of split/AES knockout samples were subjected to SDS-PAGE. ab137060 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
    Immunocytochemistry/ Immunofluorescence - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)

    Immunofluorescent staining of HeLa cells labelling Amino-terminal enhancer of split/AES using ab137060 at 1/250 dilution.

  • Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
    Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
    All lanes : Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : PC3 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 21 kDa

  • Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
    Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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