Anti-alpha Tubulin antibody [RM113] - C-terminal (ab190471)
![Anti-alpha Tubulin antibody [RM113] - C-terminal (ab190471)](https://www.abcam.com/ps/products/190/ab190471/Images/ab190471-225746-anti-alpha-tubulin-antibody-rm113-c-terminal-immunofluorescence.jpg "Anti-alpha Tubulin antibody [RM113] - C-terminal (ab190471)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM113] to alpha Tubulin - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-alpha Tubulin antibody [RM113] - C-terminal
See all alpha Tubulin primary antibodies -
Description
Rabbit monoclonal [RM113] to alpha Tubulin - C-terminal -
Host species
Rabbit -
Specificity
ab190471 reacts to alpha-Tubulin, including Tubulin alpha-1A chain and Tubulin alpha-1B chain. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human alpha Tubulin (C terminal). sequence is 100% homology with Rat, Mouse, Bovine, Pig, Chick, Monkey
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Positive control
- A431 cell lysate; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab190471 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM113 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [RM113] - C-terminal (ab190471)
Immunofluorescent analysis of A431 cells labeling alpha Tubulin with ab190471 at 1/200 dilution, followed by a PE conjugated secondary antibody (red). Counterstained with DAPI (blue).
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![Western blot - Anti-alpha Tubulin antibody [RM113] - C-terminal (ab190471)](https://www.abcam.com/ps/products/190/ab190471/Images/ab190471-225745-anti-alpha-tubulin-antibody-rm113-c-terminal-western-blot.jpg)
Western blot - Anti-alpha Tubulin antibody [RM113] - C-terminal (ab190471)Anti-alpha Tubulin antibody [RM113] - C-terminal (ab190471) at 1/1000 dilution + A431 cell lysate
Predicted band size: 50 kDa
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![Anti-alpha Tubulin antibody [RM113] - C-terminal (ab190471)](https://www.abcam.com/ps/products/190/ab190471/Images/ab190471-3-benefits-of-recombinant-antibodies.png)
Anti-alpha Tubulin antibody [RM113] - C-terminal (ab190471)
