Anti-Alpha-synuclein antibody [LB 509] - BSA and Azide free (ab264092)
![Anti-Alpha-synuclein antibody [LB 509] - BSA and Azide free (ab264092)](https://www.abcam.com/ps/products/264/ab264092/Images/ab264092-355565-AntiAlphasynuclein-antibody-LB-509-BSA-and-Azide-free-flow-cytometry-pc12-rat.jpg "Anti-Alpha-synuclein antibody [LB 509] - BSA and Azide free (ab264092)")
Key features and details
- Mouse monoclonal [LB 509] to Alpha-synuclein - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Rat, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Alpha-synuclein antibody [LB 509] - BSA and Azide free
See all Alpha-synuclein primary antibodies -
Description
Mouse monoclonal [LB 509] to Alpha-synuclein - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Rat
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Immunogen
Tissue, cells or virus corresponding to Alpha-synuclein. Lewy bodies purified from patients suffering dementia with Lewy bodies.
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Epitope
ab27766 reacts with an epitope located in the region encoded by amino acids 115-122 of alpha-synuclein. -
Positive control
- WB: Recombinant human Alpha-synuclein protein. Flow Cyt: PC12 (NGF differentiated) cells.
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General notes
ab264092 is the PBS only version of ab27766.
This antibody clone is manufactured by Abcam.
Alpha-synuclein is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
LB 509 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-Alpha-synuclein antibody [LB 509] - BSA and Azide free (ab264092)
Image produced using the same antibody clone but in a different formulation, ab27766.
Overlay histogram showing PC12 (NGF differentiated) cells stained with ab27766 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab27766, 1µg/1x106) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was Mouse IgG1 [15-6E10A7] (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Blue laser (488nm) and 530/30 bandpass filter.
