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Anti-Alpha-synuclein antibody [LB 509] - BSA and Azide free (ab264092)

Key features and details

  • Mouse monoclonal [LB 509] to Alpha-synuclein - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Rat, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-Alpha-synuclein antibody [LB 509] - BSA and Azide free
    See all Alpha-synuclein primary antibodies

  • Description

    Mouse monoclonal [LB 509] to Alpha-synuclein - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Alpha-synuclein. Lewy bodies purified from patients suffering dementia with Lewy bodies.

  • Epitope

    ab27766 reacts with an epitope located in the region encoded by amino acids 115-122 of alpha-synuclein.

  • Positive control

    • WB: Recombinant human Alpha-synuclein protein. Flow Cyt: PC12 (NGF differentiated) cells.

  • General notes

    ab264092 is the PBS only version of ab27766.

    This antibody clone is manufactured by Abcam.

    Alpha-synuclein is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Flow Cytometry - Anti-Alpha-synuclein antibody [LB 509] - BSA and Azide free (ab264092)
    Flow Cytometry - Anti-Alpha-synuclein antibody [LB 509] - BSA and Azide free (ab264092)

    Image produced using the same antibody clone but in a different formulation, ab27766.

    Overlay histogram showing PC12 (NGF differentiated) cells stained with ab27766 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab27766, 1µg/1x106) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.

    Isotype control antibody (black line) was Mouse IgG1 [15-6E10A7] (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Blue laser (488nm) and 530/30 bandpass filter.

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