Antibodies, Proteins, Kits and Reagents for Life Science

Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [AktT308-G12] to AKT1 (phospho T308)
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-AKT1 (phospho T308) antibody [AktT308-G12]
See all AKT1 primary antibodies
Description

Rabbit monoclonal [AktT308-G12] to AKT1 (phospho T308)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human AKT1 (phospho T308). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P31749

Run BLAST with Run BLAST with
Positive control
- WB: HEK-293T and NIH/3T3 (both treated with 20%FBS) cell extracts. Flow cyt: K562 cells (treated with IFNa + IL-4 + pervanadate); NIH/3T3 cellsand C6 (both treated with PDGF).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

AktT308-G12
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)

Flow cytometric analysis of C6 cells unstained and treated with imatinib as negative control (blue) or treated with imatinib or with PDGF (green) using ab278565 at 0.01 µg/mL.
Western blot - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)

All lanes : Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565) at 0.1 µg/ml

Lane 1 : NIH3T3 cell extract treated with 20% FBS
Lane 2 : NIH3T3 cell extract untreated with 20% FBS

Predicted band size: 55 kDa
Flow Cytometry - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)

Flow cytometric analysis of NIH3T3 cells treated with LY294002+U0126+wortmanin and stained with secondary antibody as negative control (blue) or treated with LY294002+U0126+wortmain (red) or with PDGF (green) and stained using ab278565 at 0.01 µg/mL.
Western blot - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)

All lanes : Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565) at 0.1 µg/ml

Lane 1 : 293T cell extract treated with 20% FBS
Lane 2 : 293T cell extract untreated with 20% FBS

Predicted band size: 55 kDa
Flow Cytometry - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with LY294002 (red) or with IFNa + IL-4 + pervanadate (green) or LY294002 and blocked with phospho-peptide (black) or IFN+IL4+Pv and blocked with phospho peptide (gold) or LY294002 and blocked with non-phospho peptide (dark blue) or IFN+IL4+Pv and blocked with non-phospho peptide (purple) using ab278565 at 0.5 µg/mL.
Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)

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