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Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [AktT308-G12] to AKT1 (phospho T308)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AKT1 (phospho T308) antibody [AktT308-G12]
    See all AKT1 primary antibodies

  • Description

    Rabbit monoclonal [AktT308-G12] to AKT1 (phospho T308)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human AKT1 (phospho T308). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P31749

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T and NIH/3T3 (both treated with 20%FBS) cell extracts. Flow cyt: K562 cells (treated with IFNa + IL-4 + pervanadate); NIH/3T3 cellsand C6 (both treated with PDGF).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)
    Flow Cytometry - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)
    Flow cytometric analysis of C6 cells unstained and treated with imatinib as negative control (blue) or treated with imatinib or with PDGF (green) using ab278565 at 0.01 µg/mL.
  • Western blot - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)
    Western blot - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)
    All lanes : Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565) at 0.1 µg/ml

    Lane 1 : NIH3T3 cell extract treated with 20% FBS
    Lane 2 : NIH3T3 cell extract untreated with 20% FBS

    Predicted band size: 55 kDa

  • Flow Cytometry - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)
    Flow Cytometry - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)
    Flow cytometric analysis of NIH3T3 cells treated with LY294002+U0126+wortmanin and stained with secondary antibody as negative control (blue) or treated with LY294002+U0126+wortmain (red) or with PDGF (green) and stained using ab278565 at 0.01 µg/mL.
  • Western blot - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)
    Western blot - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)
    All lanes : Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565) at 0.1 µg/ml

    Lane 1 : 293T cell extract treated with 20% FBS
    Lane 2 : 293T cell extract untreated with 20% FBS

    Predicted band size: 55 kDa

  • Flow Cytometry - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)
    Flow Cytometry - Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)
    Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with LY294002 (red) or with IFNa + IL-4 + pervanadate (green) or LY294002 and blocked with phospho-peptide (black) or IFN+IL4+Pv and blocked with phospho peptide (gold) or LY294002 and blocked with non-phospho peptide (dark blue) or IFN+IL4+Pv and blocked with non-phospho peptide (purple) using ab278565 at 0.5 µg/mL.
  • Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)
    Anti-AKT1 (phospho T308) antibody [AktT308-G12] (ab278565)

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