Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AIP antibody [EPR13585] (ab192024)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13585] to AIP
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AIP antibody [EPR13585]
    See all AIP primary antibodies

  • Description

    Rabbit monoclonal [EPR13585] to AIP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat, HepG2, C6, Raw264.7 and NIH/3T3 cell lysates; Human thymus, Mouse brain, Mouse heart, Mouse kidney, Mouse spleen, Rat heart and Rat kidney lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13585

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    All lanes : Anti-AIP antibody [EPR13585] (ab192024) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : AIP knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab192024 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab192024 Anti-AIP antibody [EPR13585] was shown to specifically react with AIP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265502 (knockout cell lysate ab257822) was used. Wild-type and AIP knockout samples were subjected to SDS-PAGE. ab192024 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    All lanes : Anti-AIP antibody [EPR13585] (ab192024) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Human thymus cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    Western blot - Anti-AIP antibody [EPR13585] (ab192024)
    All lanes : Anti-AIP antibody [EPR13585] (ab192024) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat kidney lysate
    Lane 7 : C6 cell lysate
    Lane 8 : Raw 264.7 cell lysate
    Lane 9 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking/dilution buffer: 5% NFDM /TBST.

  • Anti-AIP antibody [EPR13585] (ab192024)
    Anti-AIP antibody [EPR13585] (ab192024)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-AIP antibody [EPR13585] (ab192024)

© 2021 Astana Biomed Group LLP. All rights reserved.