Anti-AIDA antibody [EPR19161] - BSA and Azide free (ab224718)
![Anti-AIDA antibody [EPR19161] - BSA and Azide free (ab224718)](https://www.abcam.com/ps/products/224/ab224718/Images/ab224718-285859-C1orf80WB1.jpg "Anti-AIDA antibody [EPR19161] - BSA and Azide free (ab224718)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19161] to AIDA - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Overview
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Product name
Anti-AIDA antibody [EPR19161] - BSA and Azide free
See all AIDA primary antibodies -
Description
Rabbit monoclonal [EPR19161] to AIDA - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Immortalized wild type MEF cell lysate.
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General notes
Ab224718 is the carrier-free version of ab199323. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab224718 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19161 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AIDA antibody [EPR19161] - BSA and Azide free (ab224718)All lanes : Anti-AIDA antibody [EPR19161] (ab199323) at 1/500 dilution
Lane 1 : Immortalized wild type MEF (mouse embryonic fibroblast cell line), whole cell lysate
Lane 2 : Aida knockout immortalized MEF, whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking buffer: 5% NFDM/TBST; Dilution buffer: 5% BSA/TBST.
The data was kindly provided by our collaborator Dr. Sheng-Cai Lin, Xiamen University.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199323).
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![Western blot - Anti-AIDA antibody [EPR19161] - BSA and Azide free (ab224718)](https://www.abcam.com/ps/products/224/ab224718/Images/ab224718-331001-ab199323285858C1orf80WB2.jpg)
Western blot - Anti-AIDA antibody [EPR19161] - BSA and Azide free (ab224718)All lanes : Anti-AIDA antibody [EPR19161] (ab199323) at 1/500 dilution
Lane 1 : Wild type mouse lung homogenate
Lane 2 : Aida knockout mouse lung homogenate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking buffer: 5% NFDM/TBST; Dilution buffer: 5% BSA/TBST.
The data was kindly provided by our collaborator Dr. Sheng-Cai Lin, Xiamen University.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199323).
-
![Anti-AIDA antibody [EPR19161] - BSA and Azide free (ab224718)](https://www.abcam.com/ps/products/224/ab224718/Images/ab224718-3-benefits-of-recombinant-antibodies.png)
Anti-AIDA antibody [EPR19161] - BSA and Azide free (ab224718)
