Anti-AIDA antibody [EPR20903-53] (ab234419)
![Anti-AIDA antibody [EPR20903-53] (ab234419)](https://www.abcam.com/ps/products/234/ab234419/Images/ab234419-410978-anti-aida-antibody-epr2090353-western-blot-embryonic.jpg "Anti-AIDA antibody [EPR20903-53] (ab234419)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20903-53] to AIDA
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-AIDA antibody [EPR20903-53]
See all AIDA primary antibodies -
Description
Rabbit monoclonal [EPR20903-53] to AIDA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MEF, NIH/3T3, RAE 264.7, C2C12, HeLa, U-87 MG, C6 and PC-12 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20903-53 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AIDA antibody [EPR20903-53] (ab234419)All lanes : Anti-AIDA antibody [EPR20903-53] (ab234419) at 1/1000 dilution
Lane 1 : Wild-type MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
Lane 2 : AIDA knockout MEF whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 5 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 6 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 7 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 8 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 9 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab234419 was shown to specifically react with AIDA in wild-type MEF cells as signal was lost in AIDA knockout cells. Wild-type and AIDA knockout samples were subjected to SDS-PAGE. ab234419 and ab52901 (Rabbit anti-beta Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50, 000 dilution and 1/100,000 dilution for 1 hour at room temperature before imaging respectively. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
AIDA knockout MEF cell lysate was kindly provided by our collaborator.
Exposure time: 3 minutes.
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![Anti-AIDA antibody [EPR20903-53] (ab234419)](https://www.abcam.com/ps/products/234/ab234419/Images/ab234419-2-benefits-of-recombinant-antibodies.png)
Anti-AIDA antibody [EPR20903-53] (ab234419)