Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20903-53] to AIDA - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AIDA antibody [EPR20903-53] - BSA and Azide free
    See all AIDA primary antibodies

  • Description

    Rabbit monoclonal [EPR20903-53] to AIDA - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MEF, NIH/3T3, RAE 264.7, C2C12, HeLa, U-87 MG, C6 and PC-12 whole cell lysates.

  • General notes

    ab274421 is the carrier-free version of ab234419. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab274421 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20903-53

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)
    Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)
    All lanes : Anti-AIDA antibody [EPR20903-53] (ab234419) at 1/1000 dilution

    Lane 1 : Wild-type MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
    Lane 2 : AIDA knockout MEF whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 5 : C2C12 (mouse myoblasts myoblast) whole cell lysate
    Lane 6 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 7 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
    Lane 8 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 9 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 35 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab234419, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    ab234419 was shown to specifically react with AIDA in wild-type MEF cells as signal was lost in AIDA knockout cells. Wild-type and AIDA knockout samples were subjected to SDS-PAGE. ab234419 and ab52901 (Rabbit anti-beta Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50, 000 dilution and 1/100,000 dilution for 1 hour at room temperature before imaging respectively. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    AIDA knockout MEF cell lysate was kindly provided by our collaborator.

    Exposure time: 3 minutes.

  • Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)
    Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)

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