Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AIDA antibody [EPR19161] (ab199323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19161] to AIDA
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse

Overview

  • Product name

    Anti-AIDA antibody [EPR19161]
    See all AIDA primary antibodies

  • Description

    Rabbit monoclonal [EPR19161] to AIDA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Immortalized wild type MEF cell lysate; Mouse wild type lung homogenate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19161

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-AIDA antibody [EPR19161] (ab199323)
    Western blot - Anti-AIDA antibody [EPR19161] (ab199323)
    All lanes : Anti-AIDA antibody [EPR19161] (ab199323) at 1/500 dilution

    Lane 1 : Immortalized wild type MEF (mouse embryonic fibroblast cell line), whole cell lysate
    Lane 2 : Aida knockout immortalized MEF, whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking buffer: 5% NFDM/TBST; Dilution buffer: 5% BSA/TBST.

    The data was kindly provided by our collaborator Dr. Sheng-Cai Lin, Xiamen University.

  • Western blot - Anti-AIDA antibody [EPR19161] (ab199323)
    Western blot - Anti-AIDA antibody [EPR19161] (ab199323)
    All lanes : Anti-AIDA antibody [EPR19161] (ab199323) at 1/500 dilution

    Lane 1 : Wild type mouse lung homogenate
    Lane 2 : Aida knockout mouse lung homogenate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking buffer: 5% NFDM/TBST; Dilution buffer: 5% BSA/TBST.

    The data was kindly provided by our collaborator Dr. Sheng-Cai Lin, Xiamen University.

  • Anti-AIDA antibody [EPR19161] (ab199323)
    Anti-AIDA antibody [EPR19161] (ab199323)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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