Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

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Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22395-72] to DC-SIGN + DC-SIGNR
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Reacts with: Human

Product name

Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72]
See all DC-SIGN+CD299 primary antibodies
Description

Rabbit monoclonal [EPR22395-72] to DC-SIGN + DC-SIGNR
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Recombinant Human DC-SIGN/CD209 Protein (aa59-404) and Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein (aa73-376). Human lymph node, skin and thymus tissue lysate. IHC-P: Human liver, tonsil and skin tissue. ICC/IF: THP-1 cells. Flow: THP-1 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22395-72
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling DC-SIGN + DC-SIGNR with ab245115 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining on dendritic cell-like cells of human skin (PMID: 11859097; PMID: 11859097) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

All lanes : Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115) at 1/1000 dilution

Lane 1 : Human lymph node tissue lysate
Lane 2 : Human skin tissue lysate
Lane 3 : Human thymus tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.

Exposure time:

Lane 1:3 seconds;

Lane 2:92 seconds;

Lane 3:37 seconds

Blocking and dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling DC-SIGN + DC-SIGNR with ab245115 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining on dendritic cell-like cells of human tonsil (PMID: 11859097) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling DC-SIGN + DC-SIGNR with ab245115 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining on sinusoidal endothelial cells of human liver (PMID: 16816373) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling DC-SIGN + DC-SIGNR with ab245115 at 1/100 dilution, followed by a AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) seconday antibody (green) at 1/1000 dilution.

Confocal image showing cytoplasmic and membranous staining in THP-1 cells treated with 10 ng/ml PMA for 18 hours, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN expression is induced by PMA plus IL4 in THP-1(PMID: 15070901; PMID: 22675249). DC-SIGNR/CD299 expression is induced by PMA in THP-1 (PMID 30077333). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889)(red) at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
Immunoprecipitation - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

DC-SIGN+DC-SIGNR was immunoprecipitated from 0.35 mg human tonsil tissue lysate using ab245115 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab245115 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

Lane 1: Human tonsil tissue lysate 10 μg (input).
Lane 2: ab245115 IP in Human tonsil tissue.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245115 in Human tonsil lysate. (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Flow Cytometry - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

Flow cytometric analysis of unfixed non-permeabilized THP-1 (human monocytic leukemia cell line) cells treated with 10 ng/ml PMA for 18 hours, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN+DC-SIGNR was stained in treated (red) and untreated (green) cells using ab245115 at 1/600 dilution as compared to a Rabbit monoclonal IgG (ab172730, black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody / blue). The secondary antibody was a Goat anti rabbit IgG (Dylight^® 488, ab98462) used at 1/2000 dilution. Gated on viable cells. DC-SIGN expression is induced by PMA plus IL4 in THP-1 (PMID: 15070901; PMID: 22675249). DC-SIGNR/CD299 expression is induced by PMA in THP-1 (PMID 30077333).
Western blot - Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

All lanes : Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115) at 1/1000 dilution

Lane 1 : Recombinant Human DC-SIGN/CD209 Protein (aa59-404) 20 ng
Lane 2 : Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein (aa73-376) 20 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 48,66 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking and dilution buffer: 5% NFDM/TBST
Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Images

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