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Immunology Immunoglobulins Class Switching

Anti-AICDA antibody [5G9] (ab252813)

Anti-AICDA antibody [5G9] (ab252813)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [5G9] to AICDA
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-AICDA antibody [5G9]
    See all AICDA primary antibodies
  • Description

    Rat monoclonal [5G9] to AICDA
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human AICDA. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: NAMALWA, Raji and WEHI-231 whole cell lysate. Flow Cyt: Ramos cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    5G9
  • Isotype

    IgG2b
  • Research areas

    • Immunology
    • Immunoglobulins
    • Class Switching
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

Images

  • Western blot - Anti-AICDA antibody [5G9] (ab252813)
    Western blot - Anti-AICDA antibody [5G9] (ab252813)
    All lanes : Anti-AICDA antibody [5G9] (ab252813) at 1.264 µg/ml

    Lane 1 : NAMALWA (human Burkitts lymphoma B lymphocyte), whole cell lysate
    Lane 2 : Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 4 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/2000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: K562 (PMID: 27217538).

    Exposure time: 59 seconds.

  • Flow Cytometry - Anti-AICDA antibody [5G9] (ab252813)
    Flow Cytometry - Anti-AICDA antibody [5G9] (ab252813)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / Ramos (human Burkitt's lymphoma B lymphocyte, Right) cells labelling AICDA with ab252813 at 0.316 µg/mL (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody. Negative control: K-562.

  • Anti-AICDA antibody [5G9] (ab252813)
    Anti-AICDA antibody [5G9] (ab252813)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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