Anti-AFF4 antibody (ab75866)
Key features and details
- Rabbit polyclonal to AFF4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AFF4 antibody
See all AFF4 primary antibodies -
Description
Rabbit polyclonal to AFF4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human AFF4 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab89994) -
Positive control
- This antibody gave a positive signal in the following mouse tissue lysates: Liver; Hippocampus; and cell lysate U87-MG
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RabbitNon human primatesApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).Target
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Relevance
May play a role in transcriptional regulation. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb). -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 27125 Human
- Entrez Gene: 93736 Mouse
- Omim: 604417 Human
- SwissProt: Q9UHB7 Human
- SwissProt: Q9ESC8 Mouse
- Unigene: 519313 Human
- Unigene: 395281 Mouse
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Alternative names
- AF4/FMR2 family member 4 antibody
- AF5Q31 antibody
- Alf4 antibody
see all
Images
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Western blot - Anti-AFF4 antibody (ab75866)All lanes : Anti-AFF4 antibody (ab75866) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Hippocampus (Mouse) Tissue Lysate
Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 127 kDa
Observed band size: 127 kDa
Additional bands at: 109 kDa, 98 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References (0)
ab75866 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-AFF4 antibody (ab75866)All lanes : Anti-AFF4 antibody (ab75866) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Hippocampus (Mouse) Tissue Lysate
Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 127 kDa
Observed band size: 127 kDa
Additional bands at: 109 kDa, 98 kDa. We are unsure as to the identity of these extra bands.
