anti-Adalimumab ELISA Kit (Humira®) (ab237657)
Key features and details
- Sensitivity: 30 ng/ml
- Range: 62 ng/ml - 500 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
anti-Adalimumab ELISA Kit (Humira®)
See all Adalimumab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Overall -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
30 ng/ml -
Range
62 ng/ml - 500 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
anti-Adalimumab ELISA Kit (Humira®) (ab237657) is designed to quantify/measure the antibody against Adalimumab with high specificity and sensitivity in biological matrices.
Adalimumab (Humira®) is a recombinant human IgG1 monoclonal antibody specific for Tumor Necrosis Factor-Alpha (TNF-α) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Adalimumab specifically binds to TNF alpha and blocks its interaction with p55 and p75 cell surface TNF receptors and reduces the inflammation and subsequently improves the patient’s health. Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug. However, some patients develop unwanted immunogenicity, which leads to production of anti-drug-antibodies (ADAs) inactivating the therapeutic effects of the treatment and, in rare cases, inducing adverse effects.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Adalimumab Standard S1 1 x 1ml Adalimumab Standard S2 1 x 1ml Adalimumab Standard S3 1 x 1ml Adalimumab Standard S4 1 x 1ml Adalimumab Standard S5 1 x 1ml Adalimumab Standard S6 1 x 1ml Adalimumab Standard S7 1 x 1ml Assay Buffer 1 x 50ml Confirmation Reagent 1 x 12ml Micro ELISA Plate 1 unit Peroxidase Conjugate 1 x 12ml Plate sealers 2 units Stop Solution 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml -
Relevance
Adalimumab (Humira®) is a recombinant human IgG1 monoclonal antibody specific for Tumor Necrosis Factor-Alpha (TNF-a) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Adalimumab specifically binds to TNF alpha and blocks its interaction with p55 and p75 cell surface TNF receptors and reduces the inflammation and subsequently improves the patient’s health. Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug.
Images
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Example of Standard Curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.
