Adalimumab ELISA Kit (Humira®) (ab237641)
Key features and details
- Sensitivity: 10 ng/ml
- Range: 30 ng/ml - 1000 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
Adalimumab ELISA Kit (Humira®)
See all Adalimumab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
10 ng/ml -
Range
30 ng/ml - 1000 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Adalimumab ELISA Kit (Humira®) (ab237641) is a highly specific and sensitive kit designed for the in vitro determination of Adalimumab in biological matrices such as human serum and plasma. The density of color is proportional to the amount of Adalimumab captured from the samples and can be quantified when compared with standard curve.
Adalimumab (Humira®) is a recombinant human IgG1 monoclonal antibody specific for Tumor Necrosis Factor-Alpha (TNF-α) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Adalimumab specifically binds to TNF alpha and blocks its interaction with p55 and p75 cell surface TNF receptors and reduces the inflammation and subsequently improves the patient’s health. Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Adalimumab Standard S1 1 x 1ml Adalimumab Standard S2 1 x 1ml Adalimumab Standard S3 1 x 1ml Adalimumab Standard S4 1 x 1ml Adalimumab Standard S5 1 x 1ml Adalimumab Standard S6 1 x 1ml Adalimumab Standard S7 1 x 1ml Assay Buffer 1 x 50ml HRP-conjugate Probe 1 x 12ml Micro ELISA Plate 1 unit Plate sealers 2 units Stop Solution 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml -
Relevance
Adalimumab (Humira®) is a recombinant human IgG1 monoclonal antibody specific for Tumor Necrosis Factor-Alpha (TNF-a) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Adalimumab specifically binds to TNF alpha and blocks its interaction with p55 and p75 cell surface TNF receptors and reduces the inflammation and subsequently improves the patient’s health. Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug.
Images
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Example of Adalimumab standard curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.
