Anti-CYP7A1 antibody (ab65596)
Key features and details
- Rabbit polyclonal to CYP7A1
- Suitable for: WB
- Isotype: IgG
Overview
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Product name
Anti-CYP7A1 antibody
See all CYP7A1 primary antibodies -
Description
Rabbit polyclonal to CYP7A1This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Species reactivity
Predicted to work with: Mouse, Rat, Dog, Human, Pig, Rhesus monkey
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human CYP7A1.
Read Abcam's proprietary immunogen policy (Peptide available as ab69726.) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Compatible Secondaries
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Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
Application Abreviews Notes WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa). Target
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Function
Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. -
Tissue specificity
Detected in liver. -
Pathway
Lipid metabolism; bile acid biosynthesis. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1581 Human
- Entrez Gene: 13122 Mouse
- Entrez Gene: 25428 Rat
- Omim: 118455 Human
- SwissProt: P22680 Human
- SwissProt: Q64505 Mouse
- SwissProt: P18125 Rat
- Unigene: 1644 Human
see all -
Alternative names
- Cholesterol 7 alpha hydroxylase antibody
- Cholesterol 7 alpha monooxygenase antibody
- Cholesterol 7-alpha-hydroxylase antibody
see all
Images
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.-
Western blot - Anti-CYP7A1 antibody (ab65596)This image is courtesy of an anonymous AbreviewAll lanes : Anti-CYP7A1 antibody (ab65596) at 1/1000 dilution
All lanes : Mouse liver lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/5000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 58 kDa
Additional bands at: 40 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)Blocking: 5% Milk
References (24)
ab65596 has been referenced in 24 publications.
- Famulari ES et al. Human liver stem cells express UGT1A1 and improve phenotype of immunocompromised Crigler Najjar syndrome type I mice. Sci Rep 10:887 (2020). PubMed: 31965023
- Liu L et al. Naturally Occurring TPE-CA Maintains Gut Microbiota and Bile Acids Homeostasis via FXR Signaling Modulation of the Liver-Gut Axis. Front Pharmacol 11:12 (2020). PubMed: 32116693
- Li H et al. Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis. Nutr Metab (Lond) 17:34 (2020). PubMed: 32377219
- Kosakai T et al. Dietary fermented products using koji mold and sweet potato-shochu distillery by-product promotes hepatic and serum cholesterol levels and modulates gut microbiota in mice fed a high-cholesterol diet. PeerJ 7:e7671 (2019). PubMed: 31565580
- Albaugh VL et al. Role of Bile Acids and GLP-1 in Mediating the Metabolic Improvements of Bariatric Surgery. Gastroenterology 156:1041-1051.e4 (2019). PubMed: 30445014
Images
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Western blot - Anti-CYP7A1 antibody (ab65596) This image is courtesy of an anonymous AbreviewAll lanes : Anti-CYP7A1 antibody (ab65596) at 1/1000 dilution
All lanes : Mouse liver lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/5000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 58 kDa
Additional bands at: 40 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)Blocking: 5% Milk
