Anti-ACTL9 antibody (ab229876)
Images
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All lanes : Anti-ACTL9 antibody (ab229876) at 1/2000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa
Observed band size: 35,46 kDa why is the actual band size different from the predicted?
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ACTL9 using ab229876 at 1/100 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
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MCF7 (human breast adenocarcinoma cell line) cells stained for ACTL9 using ab229876 at 1/100 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL9 antibody (ab229876)
Paraffin-embedded human kidney tissue stained for ACTL9 using ab229876 at 1/100 dilution in immunohistochemical analysis.