Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869)
Key features and details
- Rabbit polyclonal to Activin Receptor Type IIB/ACVR2B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Activin Receptor Type IIB/ACVR2B antibody
See all Activin Receptor Type IIB/ACVR2B primary antibodies -
Description
Rabbit polyclonal to Activin Receptor Type IIB/ACVR2B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Activin Receptor Type IIB/ACVR2B. The exact sequence is proprietary. Center region.
Database link: Q13705 -
Positive control
- WB: U-87 MG, SK-N-SH and SK-N-AS whole cell lysate, Mouse cerebellum tissue extract. IHC-P: Mouse placenta tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869)All lanes : Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
Lane 3 : IMR32 (human brain neuroblast cell line) whole cell lysate
Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869)Paraffin-embedded mouse placenta tissue stained for Activin Receptor Type IIB/ACVR2B using ab272869 at a 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869)Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869) at 1/1000 dilution + Mouse cerebellum tissue at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody7.5% SDS-PAGE
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Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869)All lanes : Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869) at 1/50000 dilution
Lane 1 : Non-transfected (–) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected (+) HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE