Anti-6X His tag® antibody (ab137839)
Key features and details
- Rabbit polyclonal to 6X His tag®
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
Rabbit polyclonal to 6X His tag® -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
A synthetic peptide: HHHHHH (6-His) conjugated to KLH.
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Positive control
- ab2431 E. coli Positive Control (Escherichia coli) Whole Cell Lysate - expressing 6X His tag protein. His-tagged recombinant proteins.
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-6X His tag® antibody (ab137839) at 1/1000 dilution
Lane 1 : Non-transformed E. Coli lysate
Lane 2 : Non-transformed E. Coli lysate, induced
Lane 3 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
Lane 4 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced
Lysates/proteins at 10 µg per lane.
12% SDS PAGE -
All lanes : Anti-6X His tag® antibody (ab137839)
Lane 1 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
Lane 2 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced
Lysates/proteins at 10 µg per lane.
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His-tagged protein was immunoprecipitated from transfected cells using 8 ug of ab137839. The precipitated His-tagged protein was detected by ab137839 diluted at 1/500.