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Anti-6X His tag® antibody (ab137839)

Price and availability

301 536 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-6X His tag® antibody (ab137839)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to 6X His tag®
  • Suitable for: WB, IP
  • Reacts with: Species independent
  • Isotype: IgG

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Overview

  • Product name

    Anti-6X His tag® antibody
    See all 6X His tag® primary antibodies
  • Description

    Rabbit polyclonal to 6X His tag®
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    A synthetic peptide: HHHHHH (6-His) conjugated to KLH.

  • Positive control

    • ab2431 E. coli Positive Control (Escherichia coli) Whole Cell Lysate - expressing 6X His tag protein. His-tagged recombinant proteins.
  • General notes

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • His Tag

Images

  • Western blot - Anti-6X His tag® antibody (ab137839)
    Western blot - Anti-6X His tag® antibody (ab137839)
    All lanes : Anti-6X His tag® antibody (ab137839) at 1/1000 dilution

    Lane 1 : Non-transformed E. Coli lysate
    Lane 2 : Non-transformed E. Coli lysate, induced
    Lane 3 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
    Lane 4 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced

    Lysates/proteins at 10 µg per lane.


    12% SDS PAGE
  • Western blot - Anti-6X His tag® antibody (ab137839)
    Western blot - Anti-6X His tag® antibody (ab137839)
    All lanes : Anti-6X His tag® antibody (ab137839)

    Lane 1 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
    Lane 2 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced

    Lysates/proteins at 10 µg per lane.
  • Immunoprecipitation - Anti-6X His tag® antibody (ab137839)
    Immunoprecipitation - Anti-6X His tag® antibody (ab137839)
    His-tagged protein was immunoprecipitated from transfected cells using 8 ug of ab137839. The precipitated His-tagged protein was detected by ab137839 diluted at 1/500.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-6X His tag® antibody (ab137839)

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    Anti-6X His tag® antibody (ab14923)

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    Applications: ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Anti-6X His tag® antibody [EPR20547] - BSA and Azide free (ab232492)

    Applications: ChIP, Flow Cyt, ICC, IHC-P, IP, WB

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    Applications: Flow Cyt, IHC, IP, WB

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    Anti-6X His tag® antibody [4D11] (ab5000)

    Applications: Dot, ELISA, Flow Cyt, ICC/IF, IP, WB

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    HRP Anti-6X His tag® antibody [4D11] (ab202004)

    Applications: WB

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    Anti-6X His tag® antibody [JPAR-2] (ab245114)

    Applications: Flow Cyt, IHC, IP, WB

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