Annexin V-PE Apoptosis Staining / Detection Kit (with dead cell stain) (ab273274)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Colorimetric
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
-
Product name
Annexin V-PE Apoptosis Staining / Detection Kit (with dead cell stain)
See all Annexin V/ANXA5 kits -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Assay duration
Multiple steps standard assay -
Product overview
Annexin V-PE Apoptosis Staining / Detection Kit (with dead cell stain) (ab273274) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface.
Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need of fixation.
This kit includes annexin V-PE, SYTOX green dye and binding buffer. The SYTOX green dye is impermeable to live cells and apoptotic cells but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-PE and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence.
These populations can easily be distinguished by Fluorescence microscopy using FITC and PE filters or by flow cytometry using the FL1 channel (Ex= 488 nm/Em= 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-PE (Ex= 488 nm/Em= 578 nm).
-
Tested applications
Suitable for: Flow Cytmore details -
Platform
Flow cytometer
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 25 tests Annexin V-PE 1 x 125µl Binding Buffer 1 x 12.5ml SYTOX Green Dye 1 x 25µl -
Research areas
-
Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
-
Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all