alpha-L-Fucosidase Assay Kit (ab272525)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate
Overview
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Product name
alpha-L-Fucosidase Assay Kit -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Range
1 mU/ml - 100 mU/ml -
Product overview
alpha-L-Fucosidase Assay Kit (ab272525) is a non-radioactive, colorimetric assay based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
High sensitivity and wide linear range. Linear detection range (10 µL sample): 1 to 100 U/L for a 20 minute reaction.
Homogeneous and simple procedure. Simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with highthroughput liquid handling instruments. Can be readily automated to measure thousands of samples per day.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Standard (12.5 mM Nitrophenol) 1 x 1ml Stop Reagent 1 x 12ml Substrate Buffer 1 x 10ml -
Research areas
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Cellular localization
FUCA1: Lysosome. FUCA2: Secreted.
Images
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Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example Standard Curve.
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Activity Titration CurveExample activity titration curve.