Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3200] to CUL-4A + CUL-4B
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CUL-4A + CUL-4B antibody [EPR3200]
    See all CUL-4A + CUL-4B primary antibodies

  • Description

    Rabbit monoclonal [EPR3200] to CUL-4A + CUL-4B

  • Host species

    Rabbit

  • Specificity

    Due to the close homology between Cullin 4A and 4B this antibody is likely to react with both forms.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CUL-4A + CUL-4B aa 850-950. The exact sequence is proprietary.

  • Positive control

    • Fetal uterus, HeLa, A375 and fetal testis lysates and human brain tissue

  • General notes

     

     

     This product was previously labelled as Cullin 4a, Cullin 4A/B

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3200

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)
    Western blot - Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)
    All lanes : Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470) at 1/5000 dilution

    Lane 1 : Fetal uterus lysate
    Lane 2 : Hela lysate
    Lane 3 : A375 lysate
    Lane 4 : Fetal testis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 102 kDa
    Observed band size: 102,88 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)

    ab76470 at 1/100 dilution staining Cullin 4B in human brain tissue, using a HRP/AP polymerized secondary antibody.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)
    Anti-CUL-4A + CUL-4B antibody [EPR3200] (ab76470)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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