Alexa Fluor® 647 Anti-IRF5 antibody [EPR17067] (ab209034)
![Alexa Fluor® 647 Anti-IRF5 antibody [EPR17067] (ab209034)](https://www.abcam.com/ps/products/209/ab209034/Images/ab209034-256423-anti-irf5-antibody-epr17067-alexa-fluor-647-immunofluorescence.jpg "Alexa Fluor® 647 Anti-IRF5 antibody [EPR17067] (ab209034)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR17067] to IRF5
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-IRF5 antibody [EPR17067]
See all IRF5 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR17067] to IRF5 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Mouse
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Immunogen
Recombinant fragment within Human IRF5 aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13568-4 -
Positive control
- ICC/IF: Raw264.7 cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17067 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-IRF5 antibody [EPR17067] (ab209034)
ab209034 staining IRF5 in RAW264.7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209034 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-IRF5 antibody [EPR17067] (ab209034)](https://www.abcam.com/ps/products/209/ab209034/Images/ab209034-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-IRF5 antibody [EPR17067] (ab209034)
