Anti-IRF5 antibody (ab245475)
Key features and details
- Rabbit polyclonal to IRF5
- Suitable for: IP, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-IRF5 antibody
See all IRF5 primary antibodies -
Description
Rabbit polyclonal to IRF5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse -
Immunogen
Synthetic peptide within Mouse IRF5 aa 175-225. The exact sequence is proprietary.
Database link: P56477 -
Positive control
- WB: Mouse bone marrow dendritic cell lysate. IP: Mouse bone marrow dendritic cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab245475 was affinity purified using an epitope specific to IRF5 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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IRF5 was immunoprecipitated from 1 mg (20% of IP loaded) mouse bone marrow dendritic cell lysate using ab245475 at 6 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab245475 at 1 µg/ml.
Lane 1: ab245475 IP in mouse bone marrow dendritic cell lysate.
Lane 2: Control IgG in mouse bone marrow dendritic cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
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All lanes : Anti-IRF5 antibody (ab245475) at 0.04 µg/ml
Lane 1 : Mouse bone marrow dendritic cell lysate at 50 µg
Lane 2 : Mouse bone marrow dendritic cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 3 minutes