Aldehyde Assay Kit (Fluorometric) (ab138882)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture media, Cell Lysate, Plasma, Saliva, Urine
- Sensitivity: 3 µM
Overview
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Product name
Aldehyde Assay Kit (Fluorometric)
See all Aldehyde kits -
Detection method
Fluorescent -
Sample type
Saliva, Urine, Plasma, Cell Lysate, Cell culture media -
Assay type
Quantitative -
Sensitivity
3 µM -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Aldehyde Quantification Assay Kit (Fluorometric) (ab138882) is used for quantifying aldehydes at higher pH by using a proprietary fluorogenic dye that generates a strongly fluorescent product upon reacting with an aldehyde. This fluorimetric kit provides a sensitive mix-and-read method to detect as little as 0.3 nanomole of aldehyde/100 µL assay volume (3 µM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be read by a fluorescence microplate reader at Ex/Em = 365/435 nm.
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Notes
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years. Rapid and accurate measurement of aldehydes is an important task for biological research, chemical research, food industry and environmental pollution surveillance. There are a few reagents or assay kits available for quantifying the number of aldehydes. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Aldehyde Blue Indicator 1 vial Aldehyde Reaction Buffer 1 x 6ml Aldehyde Standard 1 vial Assay Buffer 1 x 30ml DMSO 1 x 100µl -
Research areas
Images
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Aldehyde Quantification Assay Kit (ab138882)
Aldehyde levels measured in cell supernatants (μM). Samples were diluted 2 fold.
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Aldehyde Quantification Assay Kit (ab138882)Aldehyde levels measured in cell lysates showing quantity (µmol) per 1 mln cells.
Samples with the concentration of 1e8 cells/mL (HepG2) and 1.28e8 cells/mL (HeLa) were used. Samples were diluted 100 fold.
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Aldehyde Quantification Assay Kit (ab138882)Aldehyde levels measured in biological fluids (μM). Samples were diluted 1-10 fold.
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Aldehyde Quantification Assay Kit (ab138882)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Sample Standard Curve for AldehydeAldehyde dose response was measured in a solid black 96-well plate with ab138882 using a fluorescence microplate reader. As low as 3 µM of aldehyde can be detected with 15 minutes incubation (n=3).
