Alcohol dehydrogenase Assay Kit (ab272518)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Cell Lysate, Tissue Lysate
Overview
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Product name
Alcohol dehydrogenase Assay Kit
See all Alcohol Dehydrogenase kits -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Range
0.4 mU/ml - 80 mU/ml -
Product overview
Alcohol Dehydrogenase Assay Kit (ab272518) is a non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Fast and Sensitive: Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer 1 x 10ml Calibrator 1 x 1.5ml Diaphorase 1 x 120µl MTT Solution 1 x 1.5ml NAD Solution 1 x 1ml Substrate (10% Ethanol) 1 x 1ml -
Research areas
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Relevance
Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation. -
Cellular localization
Cytoplasmic -
Alternative names
- ADH
- ADH alpha subunit
- ADH beta subunit
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Images
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ADH titration curves
Typical titration curves – data provided for demonstration purposes only.
Example of Alcohol Dehydrogenase titration curves.
OD565 readings (left) and calculated activity (right).