Aldehyde Assay Kit (Colorimetric) (ab112113)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Cell culture extracts, Plasma, Saliva, Suspension cells
Overview
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Product name
Aldehyde Assay Kit (Colorimetric)
See all Aldehyde kits -
Detection method
Colorimetric -
Sample type
Saliva, Plasma, Cell culture extracts, Adherent cells, Suspension cells -
Assay type
Quantitative -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Aldehyde Assay Kit (Colorimetric) ab112113 is a a sensitive, one-step colorimetric assay to detect as little as 1 nanomole of aldehyde in a 100 µL assay volume (10 µM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step.
The aldehyde assay protocol uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS.
The signal is read with an absorbance microplate reader at 405 or 550 nm.
Aldehyde assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30-60 min
- analyze with a microplate reader -
Notes
Previously called Aldehyde Quantification Assay Kit (Colorimetric).
This kit has been used for monitoring activities of oxidases that convert an amino group to an aldehyde group.
ab112113 should be stored desiccated.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Aldehyde Standard 1 vial Assay Buffer 1 x 10ml Dilution Buffer 1 x 20ml Yellow Indicator 2 units -
Research areas
Images
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Functional Studies - Aldehyde Quantification Assay Kit (Colorimetric) (ab112113)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Aldehyde Quantification Assay Kit (Colorimetric) (ab112113)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Aldehyde Quantification Assay Kit (Colorimetric) (ab112113)Aldehyde measured in biological fluids showing quantity (uM) per mL of tested sample. Values calculated based on the 550 nm Standard Curve. Samples diluted 1-10 fold.
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Functional Studies - Aldehyde Quantification Assay Kit (Colorimetric) (ab112113)Aldehyde measured in cell culture supernatants showing quantity (uM) per mL of tested sample. Values calculated based on the 405 nm Standard Curve. Samples diluted 2 fold.
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Functional Studies - Aldehyde Quantification Assay Kit (Colorimetric) (ab112113)Aldehyde measured in cell lysates showing quantity (uM) per 1 mln cells. Values calculated based on the 550 nm Standard Curve.
Samples with the concentration of 1.26e8 cells/mL (HeLa) and 1e8 cells/mL (HepG2) were used. Samples were diluted 3 fold.
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Functional Studies - Aldehyde Quantification Assay Kit (Colorimetric) (ab112113)Aldehyde dose response was measured in a 96-well black plate with ab112113. As low as 10 µM (1 nanomol/well) of aldehyde can be detected with 30 minutes incubation (n=3). Standard curve was read at 405 nm.
