Anti-EIF3C antibody (ab19360)
Key features and details
- Rabbit polyclonal to EIF3C
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EIF3C antibody
See all EIF3C primary antibodies -
Description
Rabbit polyclonal to EIF3C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
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Immunogen
Synthetic peptide corresponding to Human EIF3C aa 850-913 (C terminal).
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Positive control
- WB: HeLa and HEK293T cell lysates IP: HEK293T cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EIF3C antibody (ab19360)All lanes : Anti-EIF3C antibody (ab19360) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 105 kDa
Exposure time: 3 minutesDetection: Chemiluminescence
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Immunoprecipitation - Anti-EIF3C antibody (ab19360)ab19360 immunoprecipitating EIF3C in HEK293T whole cell lysate.
Lane 1 : Rabbit anti EIF3C + HEK293T whole cell lysate
Lane 2 (+): ab19360 + HEK293T whole cell lysate.
Lane 3 (-): Control IgG instead of ab19360 in HEK293T whole cell lysate.
For western blotting, ab19360 was used at 1µg/mL.
Detection: Chemiluminescence
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Western blot - Anti-EIF3C antibody (ab19360)ab19360 at 0.04
ab19360 at 0.04µg/ml for Western Blot (A and B) and at 5µg/mg for immunoprecipitation (B), staining (5, 15 or 50µg for Western blot) human HEK293, mouse C2C12 and rat L6 cells (A) and (1mg for immunoprecipitation) whole cell lysate from HEK293 cells (B). Detection was by chemiluminescence with exposure times of two to four minutes.µ g/ml for Western Blot (A and B) and at 5µ g/mg for immunoprecipitation (B), staining (5, 15 or 50µ g for Western blot) human HEK293, mouse C2C12 and rat L6 cells (A) and (1mg for immunoprecipitation) whole cell lysate from HEK293 cells (B). Detection was by chemiluminescence with exposure times of two to four minutes.
