3D Cell Culture Clearing Reagent ab243302 is part of our range of reagents that enable you to set up tissue clearing, and to clear 3D cell cultures, easily and quickly. The protocols are simple and use standard laboratory equipment, and can be used with immunostaining, fluorescent proteins and chemical dyes. Clearing is reversible so that you can treat your sample for conventional H&E or IHC staining after 3D imaging.

To learn more, please review the 3D cell culture clearing kit, tissue clearing kit, or guide to clearing tissues and 3D cell cultures. 

To help you get started with clearing 3D cell cultures, we have clearing validated antibodies. Validated antibodies are for key markers, such as NeuN, GFAP, Iba1, etc, and are tested for tissue clearing with 1 mm mouse brain sections, and for 3D cell culture clearing with neuronal spheroids and hepatic spheroids (depending on the marker).

If you want to set up clearing of 3D cell cultures or tissues on a sample type that we haven’t tested or to use your own antibodies, please consult the protocol book for the 3D cell culture clearing kit or the tissue clearing kit.

These clearing reagents were developed with Visikol Inc, who developed the Visikol® HISTO™ clearing technology which is the basis of our reagents.