Recombinant mouse PD-L1 protein (Fc Chimera Active) (ab221310)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, ELISA
Preparation and Storage
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Alternative names
- B7 H
- B7 H1
- B7 homolog 1
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Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane and Endomembrane system. - Information by UniProt
Images
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ELISA - Recombinant Mouse PD-L1 protein (Fc Chimera) (ab221310)
Immobilized Recombinant Mouse CD80 protein (Fc Chimera) (ab207101) at 5 μg/mL (100 μL/well) can bind ab221310 with a linear range of 0.16-2.5 μg/mL.
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ELISA - Recombinant Mouse PD-L1 protein (Fc Chimera) (ab221310)Immobilized ab221310 at 5 μg/mL (100 μL/well) can bind Recombinant mouse PD1 protein (Active) (Biotin) (ab246151) with a linear range of 0.313-5 μg/mL.
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SDS-PAGE - Recombinant Mouse PD-L1 protein (Fc Chimera) (ab221310)SDS-PAGE analysis of reduced ab221310 stained overnight with Coomassie Blue.
The protein migrates as 65-85 kDa on a SDS-PAGE gel under reducing conditions due to glycosylation.
