Recombinant Human PD-L1 protein (ab216206)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Tags: DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
-
Product name
Recombinant Human PD-L1 protein
See all PD-L1 proteins and peptides -
Purity
> 90 % SDS-PAGE.
Purity measured by gel filtration. -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFV HGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISY GGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWT SSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEE NHTAELVIPELPLAHPPNERTDYKDDDDK -
Predicted molecular weight
26 kDa including tags -
Amino acids
19 to 239 -
Tags
DDDDK tag C-Terminus
-
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 78% Phosphate Buffer, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
Images
-
SDS-PAGE - Recombinant Human PD-L1 protein (ab216206)
4-20% SDS-PAGE analysis of ab216206 (3 μg) with Coomassie staining.
-
Functional Studies - Recombinant Human PD-L1 protein (ab216206)Assay Conditions: 10 nM PD-1, Biotinlabeled was mixed with varying amounts of PD-L1, FLAG-tag ab216206 in PD-1 Assay Buffer for 1 hr at RT. FLAG-acceptor beads were added and incubated for 30 min at RT followed by strep-conjugated donor beads for 30 min at RT. Alpha counts were read.
-
Size Exclusion Chromatography - Recombinant Human PD-L1 protein (ab216206)Gel Filtration Curve.
